http://chlamydiae.com/twiki/bin/view/Diagnostics The Diagnostics web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise. en-us Copyright 2013 by contributing authors TWiki Administrator [info@chlamydiae.com] The contributing authors of TWiki TWiki http://chlamydiae.com/twiki/bin/view/Diagnostics http://chlamydiae.com/twiki/pub/TWiki/TWikiLogos/chlamhoechst175x50.gif http://chlamydiae.com/twiki/bin/view/Diagnostics/DiagnosticsConclusion Molecular diagnostics. Conclusions. Nobody in their right mind would want to confirm a chlamydial infection by isolation of the organism in tissue culture, if they ... (last changed by MeWard) 2011-03-14T13:41:42Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/LcrTest Molecular diagnostics : LCR, the ligase chain reaction The Abbott LCx® test. This test is now obsolete . The Abbott LCx® test, based on a ligase chain reaction ... (last changed by MeWard) 2011-03-14T13:38:37Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/PaceHybridization Molecular diagnostics. Detection of chlamydial DNA by direct hybridization. The Gen Probe Pace 2® DNA hybridization test. All nucleic acid based chlamydial diagnostic ... (last changed by MeWard) 2011-03-14T13:35:51Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/WhatUseSerology Classic diagnostics Do we need serodiagnosis? Chlamydial serology is undeniably useful for comparing sample populations for prior or current exposure to chlamydial ... (last changed by MeWard) 2011-03-14T13:35:12Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/MifSerology Classic diagnostics Micro Immunofluorescence for chlamydial antibody. The micro immunofluorescence test (MIF). The MIF test as classically performed consists of ... (last changed by MeWard) 2011-03-14T13:33:55Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/SpecimenCollection Introduction to chlamydial diagnostics Specimen collection. Female genital tract Cervical swab. The procedures are generally well known, and will be referred to ... (last changed by MeWard) 2011-03-14T13:28:37Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/DiagnosticsIntro Introduction: Laboratory diagnosis of chlamydial infections. Chlamydial infections are frequently asymptomatic. Confirmation of chlamydial infection usually depends ... (last changed by MeWard) 2011-03-14T13:24:46Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/NaatCytology Molecular diagnostics. Use of combined chlamydia testing and cervical cytology. Interest in combined cervical cancer and chlamydia screening stems from the realisation ... (last changed by MeWard) 2011-03-14T13:08:00Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/FirstTMA Molecular diagnostics. First Generation Transcription Mediated Amplification (TMA). The Gen Probe AMP CT® Fig 1. Transcription mediated amplification in pictures ... (last changed by MeWard) 2011-03-14T13:03:52Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/NucleicacidAmplificationTests Molecular diagnosis of chlamydial infections Nucleic acid amplification based tests Introduction A survey of general practitioners in England and Wales by the Public ... (last changed by MeWard) 2011-03-14T12:05:58Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/PcrTests Molecular diagnostics: PCR Roche AMPLICOR® CT/NG and Roche COBAS® AMPLICOR® CT/NG PCR, the polymerase chain reaction, was conceived by Kary Mullis in 1983 Saiki ... (last changed by MeWard) 2011-03-14T12:05:58Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/SecondTMA Molecular diagnostics The second generation TMA. The Gen Probe APTIMA Combo 2. Principles of the test. Fig 1. Second generation TMA nucleic acid amplification test ... (last changed by MeWard) 2011-03-12T22:32:13Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/StrandDisplacement Molecular diagnostics Strand displacement amplification (SDA) Introduction to the BDProbeTec®ET. A number of different amplification techniques exist see: nucleic ... (last changed by MeWard) 2011-03-12T17:45:18Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/MolecularBeacons Molecular Diagnostics Molecular beacons Introduction to beacon technology Comment. This was an article written before the introduction of the current generation ... (last changed by MeWard) 2011-03-12T16:33:15Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/PointofCareTests Classic diagnostics Point of care tests for resource limited settings. The frequent occurrence of asymptomatic infection means that chlamydial infections cannot be ... (last changed by MeWard) 2011-03-11T17:15:23Z MeWard http://chlamydiae.com/twiki/bin/view/Diagnostics/DiagnosticIF Classic diagnostics Immunofluorescence. Immunofluorescence using polyclonal antisera had been used since the 1960s for the detection of chlamydial antigen, both in ... (last changed by MeWard) 2011-03-11T12:26:36Z MeWard